Welcome to the dbGuide database, a resource containing validated guide RNA sequences that have been used in gene editing experiments in human and mouse cells.

We currently have aggregated information from over 1000 publications using the CRISPR/Cas9 technology in human or mouse cells as well as data generated by our lab assessing editing efficiency by Illumina sequencing. Each guide RNA listed that was sourced from a primary publication has a direct link to the publication.

Search with either a HUGO gene symbol (e.g. KRAS), Ensembl transcript (e.g. ENST00000256078), Ensembl gene (e.g. ENSG00000133703), genomic coordinates in BED format (e.g. chr12:25205246-25250929), or sgRNA spacer sequence (without PAM).
CAPTCHA
Point of Contact
dbGuide Help

Related Content

Artistic rendition of gene editing using CRISPR-Cas9 technology

sgRNA Scorer

Learn more
Icon

Genome Modification Core

Learn more

Submit your data

To keep this resource current, we will be actively updating this database on a monthly basis and to help facilitate this, we invite researchers to submit data into our database.

  1. Fill out our data template file.
    • Review our complete list of human and mouse sgRNA sequences in our database.
  2. Email the completed file to sgrnascorer@nih.gov.

All contributions to this database will be given proper citation to the original publication. If there are any errors where the source publication is incorrectly matched, please let us know.